Rapid identification of transgenic cotton (Gossypium hirsutum L.) plants by loop-mediated isothermal amplification
نویسندگان
چکیده
منابع مشابه
Rapid detection of infectious bursal disease by loop-mediated isothermal amplification for field analysis
Infectious bursal disease (IBD) is an immunosuppressive, acute and highly contagious illness of growing-poultry stock infected with infectious bursal disease virus (IBDV). It is common in Pakistan, causing potential economic losses throughout the year. The objective of the study is to propose a rapid, sensitive and specific diagnostic tool, and compare it with existing commonly used reverse tra...
متن کاملDisease resistance conferred by the expression of a gene encoding a synthetic peptide in transgenic cotton (Gossypium hirsutum L.) plants.
Fertile, transgenic cotton plants expressing the synthetic antimicrobial peptide, D4E1, were produced through Agrobacterium-mediated transformation. PCR products and Southern blots confirmed integration of the D4E1 gene, while RT-PCR of cotton RNA confirmed the presence of D4E1 transcripts. In vitro assays with crude leaf protein extracts from T0 and T1 plants confirmed that D4E1 was expressed ...
متن کاملLoop-mediated isothermal amplification: Beyond microbial identification
Loop-mediated isothermal amplification (LAMP) assay was introduced in the year 2000 by Notomi, as a highly sensitive, specific, and cost-effective technique for microbial identification. LAMP, a simple DNA amplification technique, with its fieldamenable nature has been used to detect a variety of pathogens including viruses, fungi, bacteria, and parasites and in most of the cases it surpasses p...
متن کاملبررسی کارایی روشLAMP (Loop-mediated isothermal DNA amplification)
مقدمه:سالمونلاها از مهمترین باکتری های بیماری و عامل تیفوئید، باکتریمی، آنتروکولیت و سالمونلوز است که از عفونت های مشترک انسان و حیوانات می باشد. بیماریهای ناشی از این عامل یک معضل بزرگ بهداشتی در جهان به خصوص در کشورهای در حال توسعه از جمله کشور ما می باشد. بنابراین تشخیص سریع، دقیق و به موقع آن برای جلوگیری از اپیدمی و عوارض مخرب این باکتری ضروری به نظر می رسد. برای تشخیص سالمونلا روش های مخت...
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ژورنال
عنوان ژورنال: Czech Journal of Genetics and Plant Breeding
سال: 2011
ISSN: 1212-1975,1805-9325
DOI: 10.17221/7/2011-cjgpb